Next generation microbioreactor for cell line development.

The innovative CYTENA C.BIRD microbioreactor technology – is the first microbioreactor that combines high-throughput culture screening, monitoring and optimisation from early stage of cell culture. It enables users to have better predictability of cell clones from day 1 and save weeks in the very cost intensive early-stage of the cell line development process. in addition, cell culture based research (e.g. synthetic biology, stem cell research and cell therapy) can potentially also benefit from C.BIRD technology.


Bringing production bioreactor conditions to 24-well and 96-well plates

The C.BIRD is a high-throughput microbioreactor for parallel cultivations in 24-well and 96-well plates that brings production bioreactor conditions to the 150 to 1600 µL scale.

Better predictability of best cell clones

The C.BIRD screens and monitors valuable cell culture parameters for hundreds of cell lines from the earliest stages, helping scientists define the best clones sooner.

Cutting weeks off the cell line development process

The C.BIRD enables early identification of the highest producing and most stable clones in order to reduce later passaging steps and efforts of current scaling-up process.

More on CYTENA

CYTENA is the leader in single-cell printing. Their patented, single cell printing technology enables fully automated isolation of single cells, with user friendly software with multiple applications including cell line development, single cell genomics and R&D. The CYTENA single cell printer (SCP) technology presents, for the first time, documented proof of clonality and provides efficient and fast single cell seeding combined with excellent cell viability.

Ellen’s take on this product


Ellen Harrington
Technical Sales Specialist

Innovative: The C.BIRD microbioreactor is designed to replace oxygen-limited static 2D cell culture with mixed 3D culture conditions earlier in the cell line development process. By bringing suspension bioreactor conditions into early stages of production, the c.bird allows for increased oxygen transfer to cells and a homogenous media environment mimicking that of large scale production stages. This results in the highest producing clones to be predicted earlier in CLD, saving time and cost.

Compact: The small size of the c.bird allows it to fit conveniently into most incubators, and is available in either vertical or horizontal orientations.

Did you know?

The c.bird is available in both 24 and 96-well plate capacities.

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    Optimise cell culture environments in 24-well and 96-well plates

    • Increase oxygen transfer rate (OTR) for both adherent cells and suspension cells
    • Bring cells into suspension culture (only for suspension cells)
    • Continuous mixing for homogenous media composition
    • Low shear rate mixing to reduce stress on cell lines

    CHO-K1 mAb expressing cell line was used for the study. The cell line was adapted to the culture suspension in a chemically defined and animal-component-free medium (CD Hybridoma medium # 11279-023 by GibCo).

    Standard 96-well plates (Eppendorf, Germany) were used for all experiments. Comparison studies were performed with cells cultivated in standard static culture and C.BIRD suspension culture in a 37°C, 5% CO2 incubator environment. Resulting cell growth and protein production were compared.

    The C.BIRD suspension culture in 96-well plates improves cell proliferation, recombinant protein yield and volume-specific productivity (Qp) compared to traditional static culture. We investigated different initial cell concentrations and measurements were done using multiple instruments to ensure accuracy. In all cases, the C.BIRD with suspension culture exhibited better performance than static culture.

    In case 1, the C.BIRD suspension culture achieved a cell density of 4.2×106 cells/ml, but the static culture only reached 1.8×106 cells/ml alter 3 days of culture. In case 2, C.BIRD suspension culture achieved a cell density of 3.1×106 cells/ml, but the static culture only reached 1.9×106 cells/ml alter 5 days of culture.

    The C.BIRD also demonstrated -2.5-3.8 times higher recombinant protein yield than static culture. (Fig. 3). For the relative volume-spe­cific productivity (Qp), C.BIRD suspension culture generated -1.8-2.6 times higher Qp than static culture.

    For the relative volume-specific productivity (Qp), the C.BIRD suspension culture generated -1.8-2.6 times higher Qp than static culture.


    CYTENA is the leader in single-cell printing.